Detection of hematopoietic stem and progenitor cells by analysis of colony-forming units

The colony-forming unit assay is performed using standardized and validated procedures. The analysis of the assay is fully automated, allowing a standardized analysis of CFUs and their photo documentation.

 

Indication:

• Quality control of cell-containing blood components and fresh or cryo-preserved stem cell specimens
• To test efficacy and optimal dosing of novel stimulatory or toxic substances (e.g. cytostatic drugs, hormones, therapeutic antibodies) with regard to proliferation and differentiation capacity of primary human cells

 

Required material: 

• ca. 0.5 – 1 ml blood of venous origin (in K2EDTA or K3EDTA; peripheral blood or cord blood, bone marrow)
• ca. 0.5 ml apheresis (in ACD)
• Cryo-preserved stem cell specimen

 

Storage and transport of material:

• <24 hours: at room temperature or 2-6 °C
• at -196 °C (liquid nitrogen)