Determination of cell numbers for vital hematopoietic stem cells by flow cytometry

All measurements are performed using CE-certified test procedures. Our lab regularly participates in interlaboratory comparisons (“Ringversuche”) to prove proper accomplishment of the necessary procedures.

 

Indication:

• Quality control of fresh or cryo-preserved stem cell specimen
• Assessment of mobilization of peripheral blood stem cells to determine the optimal time point for the stem cell apheresis procedure
• Calculation of the stem cell dose

 

Required material:

• ca. 0.5 – 1 ml blood of venous origin (in K2EDTA or K3EDTA; peripheral blood or cord blood, bone marrow)
• ca. 0.5 ml apheresis (in ACD)
• Cryo-preserved stem cell specimen

 

Storage and transport of material:

• <24 hours: at 2-6 °C
• At -196 °C (liquid nitrogen)